F490v manual

To delete one call, select Selected. To delete all calls, select All. The Silent sound profile prevents the phone from making any sounds. There are two choices for silent call alerts, Mute the phone gives only visual indication when a call is received or Vibration the phone vibrates and provides visual indication.

Video calling allows you to transmit photos and live video from your camera phone to another video calling-capable handset, as well as receive photos and live video from that handset. Both phones must be located within a 3G Network coverage area. Consult your service provider for more details. Enter the area code and phone number. Press [ ]. Your phone switches to video calling mode and initiates the call.

During the call, your phone can transmit photos or videos from your camera and receive photos or videos from the other phone. To end the call, briefly press [ ] or close the phone. From the Idle screen, press [ ] and select Contacts. Your phone switches to video calling mode.

During the video call, your phone can transmit photos or videos from your camera and receive photos or videos from the other phone during the conversation. To end your call, briefly press [ ] or close the phone. When somebody places a video call to you, the phone rings and displays the incoming Video Call image.

If necessary, open the phone. During the video call, your phone can transmit photos or videos from your camera and receive photos or videos from the other phone during the conversation see below. Your phone provides a number of features that you can use during a video call. The main options can be accessed using the on-screen menu bar. To access any of the following features, press the left or right navigation keys to highlight the desired option.

The current setting is shown above the menu bar. You can select between:. Your camera is no longer transmitting video. To update the photo, select Show Me again, followed by Freeze Me. Press the left or right navigation key to adjust the brightness and press [ ] to save the new setting.

Your video call will continue. When this option is on, you will be prompted to choose another connection method when a video call connection is not successful. When this option is on, your phone will redial automatically if a call is unable to connect or is cut off.

You can place the current call on hold at any time. You can also make another call while you have a call in progress if your network supports this service. The call is placed on hold. Enter the new number and press [ ] to dial the second call.

To finish, end each call by pressing [ ]. For example, you might wish to speak to another person in the room without the person on the call hearing you. The soft key indicator changes to Unmute and the person on the call can no longer hear you.

The other person on the call can hear you again. The speakerphone lets people near the phone participate in a call by broadcasting the voice or voices of those on the other end of the line and using the microphone to capture the voices of everyone near the phone. To use the speakerphone function during a call, press [ ].

Use the volume keys to adjust the volume. To turn off the speakerphone function during a call, press [ ]. This option allow you to turn the key tones off or on. This allows you to use the keypad without hearing annoying key tones.

Note: To communicate with answering machines or computerized telephone systems, the Send Keypad Tone option must be selected. Note: Please contact your service provider to determine if multi-party calling is supported. All call participants are listed on the screen.

To continue adding people to the call, repeat steps 2 and 3 above. Select the number of the person you wish to talk with. You can now talk privately to that person. The other participants can still converse with each other.

All the call participants can now hear each other. Highlight a participant you wish to drop and press [ ] to place a check mark beside that participant. Repeat this step as necessary. When you are finished, press the Drop soft key. The calls to the removed participant s are ended but you can continue to talk to the others.

When you want to end a multi-party call, press [ ]. You can choose to send DTMF tones after entering the entire number you need to send rather than as you press the numeric keys.

This option is helpful for entering a password or an account number when you call an automated system, like a banking service. Begin entering the letters of the name. As you type, the first entry that matches the entered letters is highlighted. To select a different contact, scroll up or down until the entry you want is highlighted. Press [ ] to open the contact information. Call Waiting lets you answer an incoming call while you have a call in progress, if your network supports this service and you have set the Call Waiting-Voice Call option to Activate.

The first call is automatically put on hold. To end the current call, press [ ]. There are several access codes used by your phone and its SIM card. These codes help protect the phone against unauthorized use. Avoid using access codes that are similar to emergency numbers, such as , to prevent accidentally dialing an emergency number. The Phone Lock feature allows you to set a password to protect your phone against unauthorized use.

It is usually supplied with the card. If you enter an incorrect PIN three times in succession, the code is disabled and cannot be used until you re-enable it by entering the PUK see below. Contact your service provider for a new card.

If you enter an incorrect PIN2 three times in succession, the code is disabled and you cannot access the functions until you re-enable it by entering the PUK2. This section explains the various text input modes for entering characters into your phone. It also describes how to use the T9 predictive text entry system to reduce the number of key strokes required for entering text. You can select all upper case, all lower case, or initial capital format. When you are in a field that allows characters to be entered, you will see the text input mode indicator near the top right corner of the display.

T9 is a predictive text input mode based on a built-in dictionary that allows you to key in characters using a single keystroke. Your phone selects the most likely word based on your keystroke combinations.

Start entering a word by pressing the numeric keys. Press each key only once for each letter. As you type, the characters on the screen change as the built-in dictionary matches your keystrokes to commonly used words. Enter all letters in the word without editing or deleting the keystrokes. If the word displayed is not correct, press [ ] to display an alternative choice.

Press [ ] to insert a space then start entering the next word. When you press [ ] on the last alternative word available, the left soft key changes to Spell. Press and hold [ ] to clear the display. The word is added to the T9 dictionary and becomes the first word for the associated key presses. Press the key labeled with the letter you want:.

Select additional letters in the same way. Note: The cursor moves to the right when you press a different key. To enter the same letter twice or a different letter on the same key, wait for the cursor to move to the right.

Number mode lets you enter numbers into a text message. Press the keypad keys corresponding to the digits you want to enter. Symbol mode lets you insert symbols and punctuation marks into text. Press and hold [ ] to access Symbol mode. Welcome to ManualMachine. We have sent a verification link to to complete your registration. Log In Sign Up. Forgot password? Enter your email address and check your inbox.

Please check your email for further instructions. Enter a new password. Table of Contents Using This Manual Using This Manual This user manual has been specially designed to guide you through the functions and features of your phone.

Important Safety Precautions Please read these guidelines before using your wireless phone. Road Safety at All Times Do not use a hand-held phone while driving; park the vehicle first. Switching Off When Refueling Do not use the phone at a refueling point service station or near fuels or chemicals.

Switching Off in Aircraft Wireless phones can cause interference. Switching Off in Hospitals Switch your phone off near medical equipment. Interference All wireless phones may be subject to interference, which could affect their performance.

Special Regulations Follow any special regulations in force in any area and always switch your phone off whenever it is forbidden to use it, or when it may cause interference or danger for example, in a hospital. Sensible Use Use the phone only in the normal position held to the ear. Emergency Call Ensure the phone is switched on and in service.

Water Resistance Your phone is not water-resistant. Keep it dry. Accessories and Batteries Use only Samsung-approved accessories and batteries. Qualified Service Only qualified service personnel may repair your phone. Your Phone Features of Your Phone Your phone is lightweight, easy-to-use and offers many valuable features. Open Views of Your Phone The following illustrations show the rest of the main elements of your phone.

Phone Mode Slide the front of the phone upward for phone operation. In text entry mode, press to change the character input type. The shortcuts are: Press Up to access the Friends page. Messages Inbox. Press Down to open the Contacts List. Display Display Layout The internal display has three main areas: Icons Text and graphics area Soft key function indicators Area Description Icons Displays various icons that indicate phone status.

Text and graphics Displays messages, menus, instructions and any area information that you enter, such as the number you are dialing. Soft key function Shows the current functions assigned to the two soft keys. Icons Shows the received signal strength.

The more bars, the stronger the signal. Appears when a call is in progress. Appears when there is no network coverage available. Appears when the phone is accessing services on a GPRS 2. Appears when the phone is accessing services on an Edge network.

Appears when an alarm is set. Appears when a new text message has been received. Appears when a new picture message has been received. Appears when a push message has been received from the WAP server. Appears when a new voice mail message has been received. Appears when a memory card is inserted. Appears when Bluetooth wireless technology is active. Appears at the bottom of the screen during a call when the earpiece is active.

Appears at the bottom of the screen during a call when the speakerphone is on. Some functions of the Right soft key are as follows. Clear Key The Clear key [ ] is used to erase or clear numbers, text, or symbols from the display. End Key The End key [ ] is used to turn your phone on and off, disconnect calls, and return to the standby mode.

Send Key The Send key [ ] is used to answer calls, dial calls, and to recall the last number s dialed, received, or missed. Speakerphone Key During a call, press [ ] to enable the speakerphone. Multitasking Menu The Multitasking key [ ] opens an application-switch window that allows you to select from a group of commonly used applications without having to exit from a currently active application.

The menu icon choices are as follows: Call icon: Allows you to make a voice call while using the handset for other tasks. To remove a microSD card from your phone: 1. Activating Your Phone Activating your phone requires several steps: inserting the SIM card, charging the battery, and turning the phone on. Removing the Battery Note: To avoid damage, unplug the travel adapter before removing the battery from the phone. Slide the battery cover off of the phone in the direction of the arrow.

Lift the battery out of the phone bottom edge first. To remove the SIM card, slide it out of the socket. To investigate the residue polymorphism of each antigenic site, we computed the sequence variability at positions that were binding determinants for selected mAbs Additional file 1 : Fig. The data showed that site 1 and site 2 were more polymorphic than site 3 and site 4. Additionally, residues at positions , , , , , and , which are key recognition sites for multiple NAbs, were highly polymorphic.

For residues at which several alternate amino acids with different side chains were selected, the different substitutions did not contribute equally to NAb binding Fig. Collectively, the data suggest that different properties of amino acid substitutions, including hydrophobicity, polarity, and charge, might determine resistance in terms of requirements for interactions with mAbs. The impacts of natural mutations at antigenic sites on binding and neutralizing activities of RBD-specific NAbs.

Degree of binding reduction was defined as percentage by OD of each mutant relative to OD of RBD wildtype is represented as a heatmap from white reduction to blue no impact.

For c — e , polar interactions are indicated by yellow dashed lines. Cyan, heavy chain; light blue, light chain; gray, RBD. Key residues on RBD highlighted in orange sticks; key residues on heavy chain highlighted in cyan sticks. IC 50 values were calculated from three independent experiments.

We further purified 18 RBD single-point mutants and 2 co-mutations expressed by mammalian cells and measured their binding activity with the panel of NAbs Fig. The fold changes in the EC 50 values compared to those of the RBD wildtype were investigated, and the results were consistent with those of our preliminary mutational scan.

Moreover, the molecular mechanisms of the effects of the mutations on three well-characterized mAbs, CB6, S, and CR, were well explained by the structures Fig. To further investigate whether the binding escape mutants exhibited NAb resistance, we constructed a panel of 19 SARS-CoV-2 pseudovirus variants, including 17 single RBD mutants and 2 co-mutations, to examine their impacts on the neutralization potency of the 12 NAbs mentioned above Fig. Using our immune escape mapping strategy, we identified a natural mutant, EK, in the circulating virus that conferred resistance to a broadly reactive NAb, S, and five mutants that resulted in resistance to CB6.

These findings could inform the therapeutic use of these antibodies in clinical studies. Expectedly , the B. However, the site 3- and site 4-targeting antibodies retained their ability to neutralize the B.

The majority of the mutants retained or even exhibited enhanced hACE2 binding. These data provide a reasonable explanation for the high frequencies of the three mutations in clinical sequencing data Additional file 1 : Fig. It is possible that the mutations altered the charge within the flexible loop region of the RBM, creating a more favourable environment for binding. Overall, our data is useful for understanding the evolutionary mechanism that governs the emergence of viral escape mutants.

The binding affinity between antibody escape mutants and ACE2. To examine whether pseudoviruses with the key antigenic escape mutations conferred resistance to convalescent plasma from the first wave of SARS-CoV-2 infection in early , nine out of 36 convalescent plasma samples 2, 6, 23, 24, 25, 26, 27, 28, and 32 were selected, and they exhibited different degrees of S-binding, receptor-blocking, and neutralizing activity Additional file 1 : Table.

The resistance profile of each human convalescent plasma sample was distinct, possibly because of the different repertoires of antigenic sites on the RBD targeted by polyclonal antibodies Fig. Markedly, KN, FL, LR, EK, and RS resulted in resistance to at least six plasma samples, as the NAb titers were approximately 2—4 times lower than those for the wildtype, indicating that NAbs targeting these key residues were enriched in human convalescent plasma.

On the other hand, both FS and NK resulted in resistance to neutralization by plasma samples 6 and 24; in particular, the NAb titer of plasma sample 24 against FS was reduced by 4.

As expected, the single EQ substitution had a milder impact than EK. We observed that the substitution NY and SN was neutralized in the same level as the wildtype by the majority of the plasma samples, in agreement with the previous studies [ 10 , 52 ].

In addition, the combination of mutations resulted in more resistance than single mutations due to an additive effect caused by KN and EK. In contrast, the double LR and EQ mutations appeared a moderate decrease in neutralizing activity and failed to show an obvious additive effect compared to single mutation. One possible reason could be that the two key residues within antigenic 2 provide favorable interactions with the same cluster of antibodies, which were observed in our panel of mAbs L,K, and L.

Overall, these data suggest that circulating viruses with single mutations at antigen-binding hot spots could be resistant to neutralization by human convalescent plasma but that no single-amino acid mutation can enable robust escape.

The evolution of co-mutations at distinct major antigenic sites is worthy of considerable attention. Effect of natural mutations on neutralizing activity of convalescent plasma.

NT 50 values were calculated from three independent experiments. New variants of SARS-CoV-2 continue to emerge; thus, it is critical to understand how the mutations within RBD facilitate the evading of immune protection or the acquiring of enhanced virulence.

Here, functional mapping analysis revealed that the majority of RBD-specific NAbs target four antigenic sites site 1, site 2, site 3, and site 4 , which are consistent with the four structurally defined regions [ 4 , 54 , 55 ]. Molecular epidemiology analysis demonstrated that the immunodominant sites 1 and 2 are the most divergent regions, suggesting that variability in these sites is the main factor contributing to RBD antigenic evolution.

In contrast, sites 3 and 4, which are in the core region, are more conserved and present a high genetic barrier to immune resistance, providing strong support for the development of antibodies and vaccine candidates that preserve these two conserved antigenic sites. In this study, we functionally defined the key determinants that are critical for RBD-specific NAb recognition and further uncovered the numerous residues often outside of the epitopes essential for RBD-specific NAb recognition by affecting RBD folding or local antigenic conformation.

These findings provide fundamental insights into antibody recognition of RBD, highlighting the dynamic and complex nature of RBD. Some of the hot spots have also been reported as the key binding determinants for NAbs by other groups [ 9 , 56 , 57 ]; thus, one application for our epitope maps is evaluating the possible immune evasion caused by RBD mutations which may be of immediate use.

Differentiating with the previous research [ 56 , 57 , 58 ], our comprehensive escape mutation map is based on the natural substitutions of the binding hot spots identified in this study instead of mapping all mutations to the RBD, thus providing a promising means to rapidly track the key immune escape variants. Our comprehensive escape mutation map based on the natural substitutions of the binding hot spots not only confirms the widely circulating strains carrying important immune escape RBD mutations such as KN, EK, and LR, but also facilitates the identification of new immune escape-enabling mutations that are already present at clinical isolates such as FL, NK, FS, and RS.

For instance, as shown in Additional file 1 : Fig. LR and RS co-mutations are present in some strains from different lineages in particular a new lineage C. These escape mutations or co-mutations may pose a new threat to current vaccine strategies in consideration of their little cost on receptor affinity Fig.

Given the in vitro neutralization data of RBD variants present in B. Thus, special attention should be paid to the accumulation of co-mutations at distinct major antigenic sites during evolution. Comparison of the hACE2 binding affinity between RBD mutants demonstrated that some antigenic escape-enabling mutations preserved or even enhanced hACE2 binding, which implies that these mutations are not accompanied by loss of fitness and are more likely to occur and spread quickly under immune pressure.

The substitution LR was particularly notable, as it also conferred resistance to multiple NAbs and human convalescent plasma. Co-mutations are needed to balance the fitness cost of antigenic change, as has been described for other viruses [ 59 , 60 ].

Given the high mutation rate of this virus, additional combinations of mutations that are compatible with viral fitness or are associated with enhanced viral resistance are likely to arise [ 7 ]. Future work that combines investigations of antigenic mutations and receptor-binding affinity-affecting mutations should be performed to analyze antigenic changes, and research on viral fitness evolution is needed to monitor emerging high-risk strains.

Additionally, the reagents described here, particularly the three new broadly reactive NAbs, offer new options for antibody-based therapeutics and provide a useful set of tools for the evaluation of vaccines that are currently under investigation.

All data generated including all raw data are included in the main paper and its additional supporting files. A new coronavirus associated with human respiratory disease in China. Eur Ann Allergy Clin Immunol. The vaccine journey for COVID a comprehensive systematic review of current clinical trials in humans. Panminerva Med. Mapping neutralizing and immunodominant sites on the SARS-CoV-2 spike receptor-binding domain by structure-guided high-resolution serology.

Lancet Infect Dis. Cell Host Microbe. McNally A. PubMed Article Google Scholar. Nat Med. BNTb2-elicited neutralization of B. Wise J. Covid the EK mutation and the risks it poses. Rapid mapping of protein functional epitopes by combinatorial alanine scanning. Structural consequences of mutations in interfacial Tyr residues of a protein antigen-antibody complex.

J Biol Chem. Global mapping of antibody recognition of the hepatitis C virus E2 glycoprotein: implications for vaccine design. Junctional and somatic hypermutation-induced CX4C motif is critical for the recognition of a highly conserved epitope on HCV E2 by a human broadly neutralizing antibody. Cell Mol Immunol. Rapid generation of fully human monoclonal antibodies specific to a vaccinating antigen.

Nat Protoc. Nat Commun. BMC Bioinformatics. DUET: a server for predicting effects of mutations on protein stability using an integrated computational approach. Nucleic Acids Res.

Mol Biol Evol. Clustal W and Clustal X version 2. An online coronavirus analysis platform from the National Genomics Data Center. Enter the pin code to sim card 3. At the top of screen an "exclamation mark" icon will appear 6. Click on "exclamation mark" icon 7. There will appear link "settings" - click on it 8. Virtual keyboard appears on screen with Enter PIN prompt. Enter your 8-digit unlock code. Then insert a sim card that is from another network that your phone is not locked to.

An Wrong sim message will come up on the screen 4. At this time a white screen will appear which says "exit" 6. Tap the right soft key, occasionally it will not go to the next screen, if so press the down key 7. From the menu that appears scroll down to the malloc Fail option, this will reboot, and normal service screen will appear. Turn phone off, and then turn on Go to menu by tapping left soft key, then settings in bottom right hand corner, then choose option number 7 security, then choose option number 6 SIM Lock, select option 1 disable Then enter eight zeros , the message will come up sim lock disabled with a check mark, your phone is now unlocked to use with other carriers.

Non Android Phones On some non-android models you can simply insert a non accepted SIM card and let the phone prompt you to enter the unlocking code. A SIM it's not locked to However, with many non-android phones you may need to check which lock s are active on the handset first. Phone Freeze If your phone displays this message or similar when inserting an incorrect sim, you must do the following: When you insert a non-accepted SIM card and your phone displays this message or similar, you must do the following: Turn on the phone with non-accepted SIM so phone displays 'Phone Freeze, SIM unavailable.